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Krox-20 patterns the hindbrain through both cell-autonomous and non cell-autonomous mechanisms.

ABSTRACT: The Krox-20 gene encodes a zinc finger transcription factor, which has been shown previously, by targeted inactivation in the mouse, to be required for the development of rhombomeres (r) 3 and 5 in the segmented embryonic hindbrain. In the present work, Krox-20 was expressed ectopically in the developing chick hindbrain by use of electroporation. We demonstrate that Krox-20 expression is sufficient to confer odd-numbered rhombomere characteristics to r2, r4, and r6 cells, presumably in a cell-autonomous manner. Therefore, Krox-20, appears as the major determinant of odd-numbered identity within the hindbrain. In addition, we provide evidence for the existence of a non cell-autonomous autoactivation mechanism allowing recruitment of Krox-20-positive cells from even-numbered territories by neighboring Krox-20-expressing cells. On the basis of these observations, we propose that Krox-20 regulates multiple, intertwined steps in segmental patterning: Initial activation of Krox-20 in a few cells leads to the segregation, homogenization, and possibly expansion of territories to which Krox-20 in addition confers an odd-numbered identity.

SUBMITTER: Giudicelli F

PROVIDER: S-EPMC312642 | biostudies-literature | 2001 Mar

REPOSITORIES: biostudies-literature

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Krox-20 patterns the hindbrain through both cell-autonomous and non cell-autonomous mechanisms.

Giudicelli F F Taillebourg E E Charnay P P Gilardi-Hebenstreit P P

Genes & development 20010301 5

The Krox-20 gene encodes a zinc finger transcription factor, which has been shown previously, by targeted inactivation in the mouse, to be required for the development of rhombomeres (r) 3 and 5 in the segmented embryonic hindbrain. In the present work, Krox-20 was expressed ectopically in the developing chick hindbrain by use of electroporation. We demonstrate that Krox-20 expression is sufficient to confer odd-numbered rhombomere characteristics to r2, r4, and r6 cells, presumably in a cell-au ...[more]

PMID: 11238377

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Dataset Information

Krox-20 patterns the hindbrain through both cell-autonomous and non cell-autonomous mechanisms.

Publications

Krox-20 patterns the hindbrain through both cell-autonomous and non cell-autonomous mechanisms.

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