Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad.
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ABSTRACT:
SUBMITTER: Imamovic L
PROVIDER: S-EPMC3126450 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad.
Imamovic Lejla L Muniesa Maite M
Applied and environmental microbiology 20110325 10
Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis. ...[more]
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