Ontology highlight
ABSTRACT:
SUBMITTER: Jose AM
PROVIDER: S-EPMC31269 | biostudies-literature | 2001 Apr
REPOSITORIES: biostudies-literature
Jose A M AM Soukup G A GA Breaker R R RR
Nucleic acids research 20010401 7
An allosteric ribozyme that requires two different effectors to induce catalysis was created using modular rational design. This ribozyme construct comprises five conjoined RNA modules that operate in concert as an obligate FMN- and theophylline-dependent molecular switch. When both effectors are present, this 'binary' RNA switch self-cleaves with a rate enhancement of approximately 300-fold over the rate observed in the absence of effectors. Kinetic and structural studies implicate a switching ...[more]