Ontology highlight
ABSTRACT:
SUBMITTER: Jacobs JS
PROVIDER: S-EPMC3127064 | biostudies-literature | 2011 Apr-Jun
REPOSITORIES: biostudies-literature
Jacobs Julie S JS Hong Xiaojing X Eberl Daniel F DF
Fly 20110401 2
Creating designer mutations in large genes is a challenge. Size limitations imposed by site-directed mutagenesis (SDM), coupled with the paucity of unique restriction enzyme sites, make subsequent cloning of these constructs extremely difficult. "Mutagenesis via Serial Small Mismatch Recombineering" (MSSMR) combines sequential recombineering steps with SDM to create seamless, pre-specified mutations as small as a single base pair. We demonstrate the simultaneous cloning of wild type and mutant c ...[more]