Cell cytoskeleton and tether extraction.
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ABSTRACT:
SUBMITTER: Pontes B
PROVIDER: S-EPMC3127177 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Publications
Cell cytoskeleton and tether extraction.
Pontes B B Viana N B NB Salgado L T LT Farina M M Moura Neto V V Nussenzveig H M HM
Biophysical journal 20110701 1
We perform a detailed investigation of the force × deformation curve in tether extraction from 3T3 cells by optical tweezers. Contrary to conventional wisdom about tethers extracted from cells, we find that actin filaments are present within them, so that a revised theory of tether pulling from cells is called for. We also measure steady and maximum tether force values significantly higher than previously published ones for 3T3 cells. Possible explanations for these differences are investigated. ...[more]
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