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Syntrophomonadaceae-affiliated species as active butyrate-utilizing syntrophs in paddy field soil.


ABSTRACT: DNA-based stable-isotope probing was applied to identify the active microorganisms involved in syntrophic butyrate oxidation in paddy field soil. After 14 and 21 days of incubation with [U-(13)C]butyrate, the bacterial Syntrophomonadaceae and the archaeal Methanosarcinaceae and Methanocellales incorporated substantial amounts of (13)C label into their nucleic acids. Unexpectedly, members of the Planctomycetes and Chloroflexi were also labeled with (13)C by yet-unclear mechanisms.

SUBMITTER: Liu P 

PROVIDER: S-EPMC3127591 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Syntrophomonadaceae-affiliated species as active butyrate-utilizing syntrophs in paddy field soil.

Liu Pengfei P   Qiu Qiongfen Q   Lu Yahai Y  

Applied and environmental microbiology 20110401 11


DNA-based stable-isotope probing was applied to identify the active microorganisms involved in syntrophic butyrate oxidation in paddy field soil. After 14 and 21 days of incubation with [U-(13)C]butyrate, the bacterial Syntrophomonadaceae and the archaeal Methanosarcinaceae and Methanocellales incorporated substantial amounts of (13)C label into their nucleic acids. Unexpectedly, members of the Planctomycetes and Chloroflexi were also labeled with (13)C by yet-unclear mechanisms. ...[more]

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