PmoA Primers for detection of anaerobic methanotrophs.
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ABSTRACT:
SUBMITTER: Luesken FA
PROVIDER: S-EPMC3127593 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
pmoA Primers for detection of anaerobic methanotrophs.
Luesken Francisca A FA Zhu Baoli B van Alen Theo A TA Butler Margaret K MK Diaz Marina Rodriguez MR Song Bongkeun B Op den Camp Huub J M HJ Jetten Mike S M MS Ettwig Katharina F KF
Applied and environmental microbiology 20110401 11
Published pmoA primers do not match the pmoA sequence of "Candidatus Methylomirabilis oxyfera," a bacterium that performs nitrite-dependent anaerobic methane oxidation. Therefore, new pmoA primers for the detection of "Ca. Methylomirabilis oxyfera"-like methanotrophs were developed and successfully tested on freshwater samples from different habitats. These primers expand existing molecular tools for the study of methanotrophs in the environment. ...[more]
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