Detection of a secreted metalloprotease within the nuclei of liver cells.
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ABSTRACT:
SUBMITTER: Hunt RC
PROVIDER: S-EPMC3128511 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
Detection of a secreted metalloprotease within the nuclei of liver cells.
Hunt Ryan C RC Geetha S S Allen Courtni E CE Hershko Klilah K Fathke Robert R Kong Philip L PL Plum Elizabeth E Struble Evi Budo EB Soejima Kenji K Friedman Scott S Garfield Susan S Balaji S S Kimchi-Sarfaty Chava C
Molecular bioSystems 20110411 6
ADAMTS13 is a secreted zinc metalloprotease expressed by various cell types. Here, we investigate its cellular pathway in endogenously expressing liver cell lines and after transient transfection with ADAMTS13. Besides compartmentalizations of the cellular secretory system, we detected an appreciable level of endogenous ADAMTS13 within the nucleus. A positively charged amino acid cluster (R-Q-R-Q-R-Q-R-R) present in the ADAMTS13 propeptide may act as a nuclear localization signal (NLS). Fusing t ...[more]
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