Dataset Information

A dynamic and complex network regulates the heterosis of yield-correlated traits in rapeseed (Brassica napus L.).

ABSTRACT: Although much research has been conducted, the genetic architecture of heterosis remains ambiguous. To unravel the genetic architecture of heterosis, a reconstructed F(2) population was produced by random intercross among 202 lines of a double haploid population in rapeseed (Brassica napus L.). Both populations were planted in three environments and 15 yield-correlated traits were measured, and only seed yield and eight yield-correlated traits showed significant mid-parent heterosis, with the mean ranging from 8.7% (branch number) to 31.4% (seed yield). Hundreds of QTL and epistatic interactions were identified for the 15 yield-correlated traits, involving numerous variable loci with moderate effect, genome-wide distribution and obvious hotspots. All kinds of mode-of-inheritance of QTL (additive, A; partial-dominant, PD; full-dominant, D; over-dominant, OD) and epistatic interactions (additive × additive, AA; additive × dominant/dominant × additive, AD/DA; dominant × dominant, DD) were observed and epistasis, especially AA epistasis, seemed to be the major genetic basis of heterosis in rapeseed. Consistent with the low correlation between marker heterozygosity and mid-parent heterosis/hybrid performance, a considerable proportion of dominant and DD epistatic effects were negative, indicating heterozygosity was not always advantageous for heterosis/hybrid performance. The implications of our results on evolution and crop breeding are discussed.

SUBMITTER: Shi J

PROVIDER: S-EPMC3128606 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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A dynamic and complex network regulates the heterosis of yield-correlated traits in rapeseed (Brassica napus L.).

Shi Jiaqin J Li Ruiyuan R Zou Jun J Long Yan Y Meng Jinling J

PloS one 20110701 7

Although much research has been conducted, the genetic architecture of heterosis remains ambiguous. To unravel the genetic architecture of heterosis, a reconstructed F(2) population was produced by random intercross among 202 lines of a double haploid population in rapeseed (Brassica napus L.). Both populations were planted in three environments and 15 yield-correlated traits were measured, and only seed yield and eight yield-correlated traits showed significant mid-parent heterosis, with the me ...[more]

PMID: 21747942

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Project description:Rapeseed (Brassica napus L.) is an important oil crop worldwide and exhibits significant heterosis. Effective pollination control systems, which are closely linked to anther development, are a prerequisite for utilizing heterosis. The anther, which is the male organ in flowering plants, undergoes many metabolic processes during development. Although the gene expression patterns underlying pollen development are well studied in model plant Arabidopsis, the regulatory networks of genome-wide gene expression during rapeseed anther development is poorly understood, especially regarding metabolic regulations. In this study, we systematically analyzed metabolic processes occurring during anther development in rapeseed using ultrastructural observation and global transcriptome analysis. Anther ultrastructure exhibited that numerous cellular organelles abundant with metabolic materials, such as elaioplast, tapetosomes, plastids (containing starch deposits) etc. appeared, accompanied with anther structural alterations during anther development, suggesting many metabolic processes occurring. Global transcriptome analysis revealed dynamic changes in gene expression during anther development that corresponded to dynamic functional alterations between early and late anther developmental stages. The early stage anthers preferentially expressed genes involved in lipid metabolism that are related to pollen extine formation as well as elaioplast and tapetosome biosynthesis, whereas the late stage anthers expressed genes associated with carbohydrate metabolism to form pollen intine and to accumulate starch in mature pollen grains. Finally, a predictive gene regulatory module responsible for early pollen extine formation was generated. Taken together, this analysis provides a comprehensive understanding of dynamic gene expression programming of metabolic processes in the rapeseed anther, especially with respect to lipid and carbohydrate metabolism during pollen development.

Dataset Information

A dynamic and complex network regulates the heterosis of yield-correlated traits in rapeseed (Brassica napus L.).

Publications

A dynamic and complex network regulates the heterosis of yield-correlated traits in rapeseed (Brassica napus L.).

Similar Datasets

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