Ontology highlight
ABSTRACT:
SUBMITTER: Grossmann V
PROVIDER: S-EPMC3128616 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Grossmann Vera V Schnittger Susanne S Schindela Sonja S Klein Hans-Ulrich HU Eder Christiane C Dugas Martin M Kern Wolfgang W Haferlach Torsten T Haferlach Claudia C Kohlmann Alexander A
The Journal of molecular diagnostics : JMD 20110301 2
CEBPA mutations are of prognostic relevance in acute myeloid leukemia (AML) and are currently detected using a combination of denaturing high-performance liquid chromatography (DHPLC), gene scan/fragment length analysis, and direct Sanger sequencing. Next-generation deep pyrosequencing, principally, allows for the highly sensitive detection of molecular mutations. However, standard 454 chemistry laboratory procedures lack efficient amplification of guanine-cytosine (GC)-rich amplicons during the ...[more]