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Sensitive luciferin derived probes for selective carboxypeptidase activity.

ABSTRACT: Highly selective luminescent probes, QLUC-TYR and LUC-GLU, for detection of carboxypeptidase activity were synthesized. Caged substrates were first cleaved by corresponding carboxypeptidases, and then they were activated by luciferase to emit light. Enzymatic activities of biologically important carboxypeptidases can be determined using this technology.

SUBMITTER: Chang YC

PROVIDER: S-EPMC3129288 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Sensitive luciferin derived probes for selective carboxypeptidase activity.

Chang Yu-Cheng YC Chao Pei-Wen PW Tung Ching-Hsuan CH

Bioorganic & medicinal chemistry letters 20110514 13

Highly selective luminescent probes, QLUC-TYR and LUC-GLU, for detection of carboxypeptidase activity were synthesized. Caged substrates were first cleaved by corresponding carboxypeptidases, and then they were activated by luciferase to emit light. Enzymatic activities of biologically important carboxypeptidases can be determined using this technology. ...[more]

PMID: 21640584

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