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Molecular detection and identification of Rickettsia species in Ixodes pacificus in California.

ABSTRACT: We amplified 16S rRNA, gltA, and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus. While phylotype G021 has high homology to Ixodes scapularis endosymbiotic Rickettsia, phylotype G022 is a deeply branched novel spotted fever group Rickettsia.

SUBMITTER: Phan JN 

PROVIDER: S-EPMC3129645 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Molecular detection and identification of Rickettsia species in Ixodes pacificus in California.

Phan Jimmy Ninh JN   Lu Casey Roy CR   Bender William Garrett WG   Smoak Robert Marion RM   Zhong Jianmin J  

Vector borne and zoonotic diseases (Larchmont, N.Y.) 20110317 7

We amplified 16S rRNA, gltA, and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus. While phylotype G021 has high homology to Ixodes scapularis endosymbiotic Rickettsia, phylotype G022 is a deeply branched novel spotted fever group Rickettsia. ...[more]

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