Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis.
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ABSTRACT:
SUBMITTER: Ashelford K
PROVIDER: S-EPMC3129678 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis.
Ashelford Kevin K Eriksson Maria E ME Allen Christopher M CM D'Amore Rosalinda R Johansson Mikael M Gould Peter P Kay Suzanne S Millar Andrew J AJ Hall Neil N Hall Anthony A
Genome biology 20110323 3
Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype. ...[more]
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