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Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation.


ABSTRACT: Cell rearrangements shape the Drosophila embryo via spatially regulated changes in cell shape and adhesion. We show that Bazooka/Par-3 (Baz) is required for the planar polarized distribution of myosin II and adherens junction proteins and polarized intercalary behavior is disrupted in baz mutants. The myosin II activator Rho-kinase is asymmetrically enriched at the anterior and posterior borders of intercalating cells in a pattern complementary to Baz. Loss of Rho-kinase results in expansion of the Baz domain, and activated Rho-kinase is sufficient to exclude Baz from the cortex. The planar polarized distribution of Baz requires its C-terminal domain. Rho-kinase can phosphorylate this domain and inhibit its interaction with phosphoinositide membrane lipids, suggesting a mechanism by which Rho-kinase could regulate Baz association with the cell cortex. These results demonstrate that Rho-kinase plays an instructive role in planar polarity by targeting Baz/Par-3 and myosin II to complementary cortical domains.

SUBMITTER: Simoes Sde M 

PROVIDER: S-EPMC3131216 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation.

Simões Sérgio de Matos Sde M   Blankenship J Todd JT   Weitz Ori O   Farrell Dene L DL   Tamada Masako M   Fernandez-Gonzalez Rodrigo R   Zallen Jennifer A JA  

Developmental cell 20100901 3


Cell rearrangements shape the Drosophila embryo via spatially regulated changes in cell shape and adhesion. We show that Bazooka/Par-3 (Baz) is required for the planar polarized distribution of myosin II and adherens junction proteins and polarized intercalary behavior is disrupted in baz mutants. The myosin II activator Rho-kinase is asymmetrically enriched at the anterior and posterior borders of intercalating cells in a pattern complementary to Baz. Loss of Rho-kinase results in expansion of  ...[more]

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