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Proteomic analysis identified DJ-1 as a cisplatin resistant marker in non-small cell lung cancer.


ABSTRACT: The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cisplatin resistance-related proteins were identified, and DJ-1, one of the differently expressed proteins, was selected for further validation and evaluation. Immunohistochemical results demonstrated that high expression level of DJ-1 was associated with cisplatin resistance and a predictor for poor prognosis in 67 locally advanced NSCLC patients. Furthermore, in vitro results showed that silencing DJ-1 increased the proliferation inhibitory effect of cisplatin to A549/DDP cells. In conclusion, DJ-1 might play an important role in the resistibility to cisplatin, and it could also act as a novel candidate biomarker for predicting the response of NSCLC patients to cisplatin-based chemotherapy.

SUBMITTER: Zeng HZ 

PROVIDER: S-EPMC3131574 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Proteomic analysis identified DJ-1 as a cisplatin resistant marker in non-small cell lung cancer.

Zeng Hua-Zong HZ   Qu Yi-Qing YQ   Zhang Wen-Jun WJ   Xiu Bing B   Deng An-Mei AM   Liang Ai-Bin AB  

International journal of molecular sciences 20110601 6


The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cisplatin resistance-related proteins were identified, and DJ-1, one of the differently expressed proteins, was selected for further validation and evaluation. Immunohistochemical result  ...[more]

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