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Microarray-based evaluation of whole-community genome DNA amplification methods.

ABSTRACT: Three whole-community genome amplification methods, Bst, REPLI-g, and Templiphi, were evaluated using a microarray-based approach. The amplification biases of all methods were <3-fold. For pure-culture DNA, REPLI-g and Templiphi showed less bias than Bst. For community DNA, REPLI-g showed the least bias and highest number of genes, while Bst had the highest success rate and was suitable for low-quality DNA.

SUBMITTER: Wang J

PROVIDER: S-EPMC3131669 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Microarray-based evaluation of whole-community genome DNA amplification methods.

Wang Jian J Van Nostrand Joy D JD Wu Liyou L He Zhili Z Li Guanghe G Zhou Jizhong J

Applied and environmental microbiology 20110415 12

Three whole-community genome amplification methods, Bst, REPLI-g, and Templiphi, were evaluated using a microarray-based approach. The amplification biases of all methods were <3-fold. For pure-culture DNA, REPLI-g and Templiphi showed less bias than Bst. For community DNA, REPLI-g showed the least bias and highest number of genes, while Bst had the highest success rate and was suitable for low-quality DNA. ...[more]

PMID: 21498751

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