Microarray-based evaluation of whole-community genome DNA amplification methods.

ABSTRACT: Three whole-community genome amplification methods, Bst, REPLI-g, and Templiphi, were evaluated using a microarray-based approach. The amplification biases of all methods were <3-fold. For pure-culture DNA, REPLI-g and Templiphi showed less bias than Bst. For community DNA, REPLI-g showed the least bias and highest number of genes, while Bst had the highest success rate and was suitable for low-quality DNA.

SUBMITTER: Wang J 

PROVIDER: S-EPMC3131669 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature