Inhibition of glycosphingolipid biosynthesis induces cytokinesis failure.
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ABSTRACT:
SUBMITTER: Atilla-Gokcumen GE
PROVIDER: S-EPMC3131740 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Publications
Inhibition of glycosphingolipid biosynthesis induces cytokinesis failure.
Atilla-Gokcumen G E GE Bedigian A V AV Sasse S S Eggert U S US
Journal of the American Chemical Society 20110614 26
Although cells undergo dramatic shape changes during cytokinesis, the role of the plasma membrane and lipids is poorly understood. We report that inactivation of glucosyl ceramide synthase (GCS), either by RNAi or with the small molecule PPMP, causes failure of cleavage furrow ingression. Using mass-spectrometry-based global lipid profiling, we identify individual lipids that are enhanced or depleted due to GCS inhibition. We show that GCS inhibition results in the mislocalization of actin and t ...[more]
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