Ontology highlight
ABSTRACT:
SUBMITTER: Nutiu R
PROVIDER: S-EPMC3134637 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Nutiu Razvan R Friedman Robin C RC Luo Shujun S Khrebtukova Irina I Silva David D Li Robin R Zhang Lu L Schroth Gary P GP Burge Christopher B CB
Nature biotechnology 20110626 7
Several methods for characterizing DNA-protein interactions are available, but none have demonstrated both high throughput and quantitative measurement of affinity. Here we describe 'high-throughput sequencing'-'fluorescent ligand interaction profiling' (HiTS-FLIP), a technique for measuring quantitative protein-DNA binding affinity at unprecedented depth. In this approach, the optics built into a high-throughput sequencer are used to visualize in vitro binding of a protein to sequenced DNA in a ...[more]