Ontology highlight
ABSTRACT:
SUBMITTER: Wilson DJ
PROVIDER: S-EPMC3135573 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Analytical biochemistry 20110508 1
Cofactor biosynthetic pathways represent a rich source of potential antibiotic targets. The second step in biotin biosynthesis is performed by BioA, a pyridoxal 5'-phosphate (PLP)-dependent enzyme. This enzyme has been confirmed as a candidate target in Mycobacterium tuberculosis; however, the current bioassay used to measure BioA activity is cumbersome and low throughput. Here we describe the design, development, and optimization of a continuous coupled fluorescence displacement assay to measur ...[more]