Ontology highlight
ABSTRACT:
SUBMITTER: Han L
PROVIDER: S-EPMC3136278 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Han Li L Masani Shahnaz S Yu Kefei K
Proceedings of the National Academy of Sciences of the United States of America 20110627 28
Ig class-switch recombination (CSR) is directed by the long and repetitive switch regions and requires activation-induced cytidine deaminase (AID). One of the conserved switch-region sequence motifs (AGCT) is a preferred site for AID-mediated DNA-cytosine deamination. By using somatic gene targeting and recombinase-mediated cassette exchange, we established a cell line-based CSR assay that allows manipulation of switch sequences at the endogenous locus. We show that AGCT is only one of a family ...[more]