Ontology highlight
ABSTRACT:
SUBMITTER: Lan X
PROVIDER: S-EPMC3136941 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Lan Xun X Adams Christopher C Landers Mark M Dudas Miroslav M Krissinger Daniel D Marnellos George G Bonneville Russell R Xu Maoxiong M Wang Junbai J Huang Tim H-M TH Meredith Gavin G Jin Victor X VX
PloS one 20110711 7
Methyl-CpG binding domain protein sequencing (MBD-seq) is widely used to survey DNA methylation patterns. However, the optimal experimental parameters for MBD-seq remain unclear and the data analysis remains challenging. In this study, we generated high depth MBD-seq data in MCF-7 cell and developed a bi-asymmetric-Laplace model (BALM) to perform data analysis. We found that optimal efficiency of MBD-seq experiments was achieved by sequencing ∼100 million unique mapped tags from a combination of ...[more]