Ontology highlight
ABSTRACT:
SUBMITTER: Muzzey D
PROVIDER: S-EPMC3137897 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Muzzey Dale D van Oudenaarden Alexander A
Annual review of cell and developmental biology 20090101
The cloning of green fluorescent protein (GFP) 15 years ago revolutionized cell biology by permitting visualization of a wide range of molecular mechanisms within living cells. Though initially used to make largely qualitative assessments of protein levels and localizations, fluorescence microscopy has since evolved to become highly quantitative and high-throughput. Computational image analysis has catalyzed this evolution, enabling rapid and automated processing of large datasets. Here, we revi ...[more]