Proteome interrogation using nanoprobes to identify targets of a cancer-killing molecule.
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ABSTRACT:
SUBMITTER: Li L
PROVIDER: S-EPMC3138543 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
Proteome interrogation using nanoprobes to identify targets of a cancer-killing molecule.
Li Liwen L Zhang Qiu Q Liu Aifeng A Li Xiue X Zhou Hongyu H Liu Yin Y Yan Bing B
Journal of the American Chemical Society 20110415 18
We report a generic approach for identification of target proteins of therapeutic molecules using nanoprobes. Nanoprobes verify the integrity of nanoparticle-bound ligands in live cells and pull down target proteins from the cellular proteome, providing very important information on drug targets and mechanisms of action. As an example, target proteins as α-tubulin and HSP 90 have been identified and validated. ...[more]
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