Ontology highlight
ABSTRACT:
SUBMITTER: Schroder JM
PROVIDER: S-EPMC3138699 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Schrøder Jacob M JM Larsen Jesper J Komarova Yulia Y Akhmanova Anna A Thorsteinsson Rikke I RI Grigoriev Ilya I Manguso Robert R Christensen Søren T ST Pedersen Stine F SF Geimer Stefan S Pedersen Lotte B LB
Journal of cell science 20110801 Pt 15
The microtubule (MT) plus-end-tracking protein EB1 is required for assembly of primary cilia in mouse fibroblasts, but the mechanisms involved and the roles of the related proteins EB2 and EB3 in ciliogenesis are unknown. Using protein depletion experiments and expression of dominant-negative constructs we show here that EB1 and EB3, but not EB2, are required for assembly of primary cilia in cultured cells. Electron microscopy and live imaging showed that cells lacking EB1 or EB3 are defective i ...[more]