Metabolic network analysis integrated with transcript verification for sequenced genomes.
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ABSTRACT:
SUBMITTER: Manichaikul A
PROVIDER: S-EPMC3139173 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Publications
Metabolic network analysis integrated with transcript verification for sequenced genomes.
Manichaikul Ani A Ghamsari Lila L Hom Erik F Y EF Lin Chenwei C Murray Ryan R RR Chang Roger L RL Balaji S S Hao Tong T Shen Yun Y Chavali Arvind K AK Thiele Ines I Yang Xinping X Fan Changyu C Mello Elizabeth E Hill David E DE Vidal Marc M Salehi-Ashtiani Kourosh K Papin Jason A JA
Nature methods 20090713 8
With sequencing of thousands of organisms completed or in progress, there is a growing need to integrate gene prediction with metabolic network analysis. Using Chlamydomonas reinhardtii as a model, we describe a systems-level methodology bridging metabolic network reconstruction with experimental verification of enzyme encoding open reading frames. Our quantitative and predictive metabolic model and its associated cloned open reading frames provide useful resources for metabolic engineering. ...[more]
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