Ontology highlight
ABSTRACT:
SUBMITTER: Bonger KM
PROVIDER: S-EPMC3139708 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Bonger Kimberly M KM Chen Ling-chun LC Liu Corey W CW Wandless Thomas J TJ
Nature chemical biology 20110703 8
The ability to rapidly regulate the functions of specific proteins in living cells is a valuable tool for biological research. Here we describe a new technique by which the degradation of a specific protein is induced by a small molecule. A protein of interest is fused to a ligand-induced degradation (LID) domain, resulting in the expression of a stable and functional fusion protein. The LID domain is comprised of the FK506- and rapamycin-binding protein (FKBP) and a 19-amino-acid degron fused t ...[more]