Unknown

Dataset Information

0

Structure and stoichiometry of template-directed recombinant HIV-1 Gag particles.


ABSTRACT: Size polydispersity of immature human immunodeficiency virus type 1 (HIV-1) particles represents a challenge for traditional methods of biological ultrastructural analysis. An in vitro model for immature HIV-1 particles constructed from recombinant Gag proteins lacking residues 16-99 and the p6 domain assembled around spherical nanoparticles functionalized with DNA. This template-directed assembly approach led to a significant reduction in size polydispersity and revealed previously unknown structural features of immature-like HIV-1 particles. Electron microscopy and image reconstruction of these particles suggest that the Gag shell formed from different protein regions that are connected by a "scar"-an extended defect connecting the edges of two continuous, regularly packed protein layers. Thus, instead of a holey protein array, the experimental model presented here appears to consist of a continuous array of ?5000 proteins enveloping the core, in which regular regions are separated by extended areas of disorder.

SUBMITTER: Goicochea NL 

PROVIDER: S-EPMC3140650 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structure and stoichiometry of template-directed recombinant HIV-1 Gag particles.

Goicochea Nancy L NL   Datta Siddhartha A K SA   Ayaluru Murali M   Kao Cheng C   Rein Alan A   Dragnea Bogdan B  

Journal of molecular biology 20110701 4


Size polydispersity of immature human immunodeficiency virus type 1 (HIV-1) particles represents a challenge for traditional methods of biological ultrastructural analysis. An in vitro model for immature HIV-1 particles constructed from recombinant Gag proteins lacking residues 16-99 and the p6 domain assembled around spherical nanoparticles functionalized with DNA. This template-directed assembly approach led to a significant reduction in size polydispersity and revealed previously unknown stru  ...[more]

Similar Datasets

| S-EPMC2717261 | biostudies-other
| S-EPMC4111510 | biostudies-literature
| S-EPMC4795674 | biostudies-literature
| S-EPMC5270550 | biostudies-literature
| S-EPMC3184602 | biostudies-literature
| S-EPMC2977881 | biostudies-literature
| S-EPMC5848919 | biostudies-literature
| S-EPMC10698178 | biostudies-literature
| S-EPMC4079005 | biostudies-literature
| S-EPMC2777969 | biostudies-literature