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A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes.


ABSTRACT: We have developed a microfabricated biochip that enumerates CD4+ T lymphocytes from leukocyte populations obtained from human blood samples using electrical impedance sensing and immunoaffinity chromatography. T cell counts are found by obtaining the difference between the number of leukocytes before and after depleting CD4+ T cells with immobilized antibodies in a capture chamber. This differential counting technique has been validated to analyze physiological concentrations of leukocytes with an accuracy of ?9 cells per µL by passivating the capture chamber with bovine serum albumin. In addition, the counter provided T cell counts which correlated closely with an optical control (R(2) = 0.997) for CD4 cell concentrations ranging from approximately 100 to 700 cells per µL. We believe that this approach can be a promising method for bringing quantitative HIV/AIDS diagnostics to resource-poor regions in the world.

SUBMITTER: Watkins NN 

PROVIDER: S-EPMC3141315 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes.

Watkins Nicholas N NN   Sridhar Supriya S   Cheng Xuanhong X   Chen Grace D GD   Toner Mehmet M   Rodriguez William W   Bashir Rashid R  

Lab on a chip 20110201 8


We have developed a microfabricated biochip that enumerates CD4+ T lymphocytes from leukocyte populations obtained from human blood samples using electrical impedance sensing and immunoaffinity chromatography. T cell counts are found by obtaining the difference between the number of leukocytes before and after depleting CD4+ T cells with immobilized antibodies in a capture chamber. This differential counting technique has been validated to analyze physiological concentrations of leukocytes with  ...[more]

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