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Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studies.

ABSTRACT: A thorough understanding of nerve regeneration in Caenorhabditis elegans requires performing femtosecond laser nanoaxotomy while minimally affecting the worm. We present a microfluidic device that fulfills such criteria and can easily be automated to enable high-throughput genetic and pharmacological screenings. Using the 'nanoaxotomy' chip, we discovered that axonal regeneration occurs much faster than previously described, and notably, the distal fragment of the severed axon regrows in the absence of anesthetics.

SUBMITTER: Guo SX

PROVIDER: S-EPMC3143684 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature

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Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studies.

Guo Samuel X SX Bourgeois Frederic F Chokshi Trushal T Durr Nicholas J NJ Hilliard Massimo A MA Chronis Nikos N Ben-Yakar Adela A

Nature methods 20080413 6

A thorough understanding of nerve regeneration in Caenorhabditis elegans requires performing femtosecond laser nanoaxotomy while minimally affecting the worm. We present a microfluidic device that fulfills such criteria and can easily be automated to enable high-throughput genetic and pharmacological screenings. Using the 'nanoaxotomy' chip, we discovered that axonal regeneration occurs much faster than previously described, and notably, the distal fragment of the severed axon regrows in the abs ...[more]

PMID: 18408725

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