Targeted sorting of single virus-infected cells of the coccolithophore Emiliania huxleyi.
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ABSTRACT:
SUBMITTER: Martinez Martinez J
PROVIDER: S-EPMC3144233 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
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Targeted sorting of single virus-infected cells of the coccolithophore Emiliania huxleyi.
Martínez Martínez Joaquín J Poulton Nicole J NJ Stepanauskas Ramunas R Sieracki Michael E ME Wilson William H WH
PloS one 20110726 7
Discriminating infected from healthy cells is the first step to understanding the mechanisms and ecological implications of viral infection. We have developed a method for detecting, sorting, and performing molecular analysis of individual, infected cells of the important microalga Emiliania huxleyi, based on known physiological responses to viral infection. Of three fluorescent dyes tested, FM 1-43 (for detecting membrane blebbing) gave the most unequivocal and earliest separation of cells. Fur ...[more]
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