Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
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ABSTRACT:
SUBMITTER: Annibale P
PROVIDER: S-EPMC3144238 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
Annibale Paolo P Vanni Stefano S Scarselli Marco M Rothlisberger Ursula U Radenovic Aleksandra A
PloS one 20110726 7
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plas ...[more]
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