Acoustically mounted microcrystals yield high-resolution X-ray structures.
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ABSTRACT:
SUBMITTER: Soares AS
PROVIDER: S-EPMC3144476 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
Acoustically mounted microcrystals yield high-resolution X-ray structures.
Soares Alexei S AS Engel Matthew A MA Stearns Richard R Datwani Sammy S Olechno Joe J Ellson Richard R Skinner John M JM Allaire Marc M Orville Allen M AM
Biochemistry 20110509 21
We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures. ...[more]
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