Cloning and characterization of Pfl_1841, a 2-methylenebornane synthase in Pseudomonas fluorescens PfO-1.

ABSTRACT: The pfl_1841 gene from Pseudomonas fluorescens PfO-1 is the only gene in any of the three sequenced genomes of the Gram-negative bacterium Pseudomonas fluorescens that is annotated as a putative terpene synthase. The predicted Pfl_1841 protein, which harbors the two strictly conserved divalent metal binding domains found in all terpene cyclases, is closely related to several known or presumed 2-methylisoborneol synthases, with the closest match being to the MOL protein of Micromonaspora olivasterospora KY11048 that has been implicated as a 2-methylenebornane synthase. A synthetic gene encoding P. fluorescens Pfl_1841 and optimized for expression in Escherichia coli was expressed and purified as an N-terminal His(6)-tagged protein. Incubation of recombinant Pfl_1841 with 2-methylgeranyl dip

SUBMITTER: Chou WK 

PROVIDER: S-EPMC3144570 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature