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Expression, crystallization and preliminary X-ray diffraction studies of thermostable ?-1,3-xylanase from Thermotoga neapolitana strain DSM?4359.

ABSTRACT: Crystals of ?-1,3-xylanase (1,3-?-D-xylan xylanohydrolase; EC 3.2.1.32) from Thermotoga neapolitana strain DSM?4359 with maximum dimensions of 0.2×0.1×0.02?mm were grown using the sitting-drop vapour-diffusion method at 293?K over 24?h. The crystals diffracted to a resolution of 1.82?Å, allowing structure determination. The crystals belonged to space group P2(1), with unit-cell parameters a=39.061, b=75.828, c=52.140?Å; each asymmetric unit cell contained a single molecule.

SUBMITTER: Okazaki F

PROVIDER: S-EPMC3144794 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Expression, crystallization and preliminary X-ray diffraction studies of thermostable β-1,3-xylanase from Thermotoga neapolitana strain DSM 4359.

Okazaki Fumiyoshi F Ogino Chiaki C Kondo Akihiko A Mikami Bunzo B Kurebayashi Yoichi Y Tsuruta Hiroki H

Acta crystallographica. Section F, Structural biology and crystallization communications 20110630 Pt 7

Crystals of β-1,3-xylanase (1,3-β-D-xylan xylanohydrolase; EC 3.2.1.32) from Thermotoga neapolitana strain DSM 4359 with maximum dimensions of 0.2×0.1×0.02 mm were grown using the sitting-drop vapour-diffusion method at 293 K over 24 h. The crystals diffracted to a resolution of 1.82 Å, allowing structure determination. The crystals belonged to space group P2(1), with unit-cell parameters a=39.061, b=75.828, c=52.140 Å; each asymmetric unit cell contained a single molecule. ...[more]

PMID: 21795792

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