Ontology highlight
ABSTRACT:
SUBMITTER: Hiseni A
PROVIDER: S-EPMC3145076 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Applied microbiology and biotechnology 20110517 4
Two carotenoid 1,2-hydratase (CrtC) genes from the photosynthetic bacteria Rubrivivax gelatinosus and Thiocapsa roseopersicina were cloned and expressed in Escherichia coli in an active form and purified by affinity chromatography. The biochemical properties of the recombinant enzymes and their substrate specificities were studied. The purified CrtCs catalyze cofactor independently the conversion of lycopene to 1-HO- and 1,1'-(HO)(2)-lycopene. The optimal pH and temperature for hydratase activit ...[more]