Ontology highlight
ABSTRACT:
SUBMITTER: Skourti-Stathaki K
PROVIDER: S-EPMC3145960 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Skourti-Stathaki Konstantina K Proudfoot Nicholas J NJ Gromak Natalia N
Molecular cell 20110601 6
We present a molecular dissection of pause site-dependent transcriptional termination for mammalian RNA polymerase II (Pol II)-transcribed genes. We show that nascent transcripts form RNA/DNA hybrid structures (R-loops) behind elongating Pol II and are especially prevalent over G-rich pause sites positioned downstream of gene poly(A) signals. Senataxin, a helicase protein associated with AOA2/ALS4 neurodegenerative disorders, acts to resolve these R-loop structures and by so doing allows access ...[more]