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Versatile method for the detection of covalently bound substrates on solid supports by DART mass spectrometry.

ABSTRACT: Analysis of substrates directly on solid phase resins without the need for separate cleavage conditions remains an outstanding challenge in the field of solid phase synthesis. We now present the first example of simultaneous cleavage and mass spectrometric analysis of peptides from solid supports using direct analysis in real time (DART) mass spectrometry. We have shown that this method is compatible with a diverse array of solid phase resins and is suitable for analysis of both peptides and organic substrates.

SUBMITTER: Sanchez LM

PROVIDER: S-EPMC3146587 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Versatile method for the detection of covalently bound substrates on solid supports by DART mass spectrometry.

Sanchez Laura M LM Curtis Matthew E ME Bracamonte Bianca E BE Kurita Kenji L KL Navarro Gabriel G Sparkman O David OD Linington Roger G RG

Organic letters 20110624 15

Analysis of substrates directly on solid phase resins without the need for separate cleavage conditions remains an outstanding challenge in the field of solid phase synthesis. We now present the first example of simultaneous cleavage and mass spectrometric analysis of peptides from solid supports using direct analysis in real time (DART) mass spectrometry. We have shown that this method is compatible with a diverse array of solid phase resins and is suitable for analysis of both peptides and org ...[more]

PMID: 21699263

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