Ontology highlight
ABSTRACT:
SUBMITTER: Flott S
PROVIDER: S-EPMC3147262 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
EMBO reports 20110708 8
Rad51 is a key enzyme involved in DNA double-strand break repair by homologous recombination. Here, we show that in response to DNA damage, budding yeast Rad51 is phosphorylated on Ser 192 in a manner that is primarily mediated by the DNA-damage-responsive protein kinase Mec1. We show that mutating Rad51 Ser 192 to Ala or Glu confers hypersensitivity to DNA damage and homologous-recombination defects. Furthermore, biochemical analyses indicate that Ser 192 is required for Rad51 adenosine triphos ...[more]