Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.
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ABSTRACT:
SUBMITTER: Ohnishi A
PROVIDER: S-EPMC3147432 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Publications
Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.
Ohnishi Akihiro A Abe Shinko S Nashirozawa Shiho S Shimada Sayaka S Fujimoto Naoshi N Suzuki Masaharu M
Applied and environmental microbiology 20110624 15
The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates. ...[more]
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