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Detection of murine norovirus-1 by using TAT peptide-delivered molecular beacons.

ABSTRACT: A TAT peptide-delivered molecular beacon was developed and utilized to enumerate murine norovirus 1, a human norovirus (NoV) surrogate, in RAW 264.7 cells. This allowed the detection of a single infective virus within 6 h, a 12-fold improvement in time required for viral detection and quantification compared to that required by the conventional plaque assay.

SUBMITTER: Ganguli PS

PROVIDER: S-EPMC3147468 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Detection of murine norovirus-1 by using TAT peptide-delivered molecular beacons.

Ganguli Payal S PS Chen Wilfred W Yates Marylynn V MV

Applied and environmental microbiology 20110607 15

A TAT peptide-delivered molecular beacon was developed and utilized to enumerate murine norovirus 1, a human norovirus (NoV) surrogate, in RAW 264.7 cells. This allowed the detection of a single infective virus within 6 h, a 12-fold improvement in time required for viral detection and quantification compared to that required by the conventional plaque assay. ...[more]

PMID: 21652740

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