Ontology highlight
ABSTRACT:
SUBMITTER: Sluijter M
PROVIDER: S-EPMC3147543 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Journal of bacteriology 20110603 15
The RecU protein from Mycoplasma genitalium, RecU(Mge), is a 19.4-kDa Holliday junction (HJ) resolvase that binds in a nonspecific fashion to HJ substrates and, in the presence of Mn(2+), cleaves these substrates at a specific sequence (5'-G/TC↓C/TTA/GG-3'). To identify amino acid residues that are crucial for HJ binding and/or cleavage, we generated a series of 16 deletion mutants (9 N- and 7 C-terminal deletion mutants) and 31 point mutants of RecU(Mge). The point mutations were introduced at ...[more]