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Importance of a hydrophobic pocket for peptide binding in lactococcal OppA.

ABSTRACT: Lactococcal oligopeptide-binding protein A (OppA) binds peptides with widely varied lengths and sequences. We previously hypothesized that a hydrophobic pocket in OppA preferentially binds a hydrophobic peptide side chain and thus determines its binding register. Two crystal structures of OppA with different nonapeptides now indeed show binding in different registers.

SUBMITTER: Berntsson RP

PROVIDER: S-EPMC3147668 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Importance of a hydrophobic pocket for peptide binding in lactococcal OppA.

Berntsson Ronnie P-A RP Thunnissen Andy-Mark W H AM Poolman Bert B Slotboom Dirk-Jan DJ

Journal of bacteriology 20110610 16

Lactococcal oligopeptide-binding protein A (OppA) binds peptides with widely varied lengths and sequences. We previously hypothesized that a hydrophobic pocket in OppA preferentially binds a hydrophobic peptide side chain and thus determines its binding register. Two crystal structures of OppA with different nonapeptides now indeed show binding in different registers. ...[more]

PMID: 21665971

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