ARF6-regulated shedding of tumor cell-derived plasma membrane microvesicles.

ABSTRACT:

Background

Increased mitogen-activated protein kinase (MAPK) signaling, small GTPase activation, cytoskeletal rearrangements, and the directed targeting of proteases to sites of extracellular matrix degradation all accompany the process of tumor cell invasion. Several studies have implicated the small GTP-binding protein ARF6 in tumor cell invasion, although the molecular basis by which ARF6 facilitates this process is unclear.

Results

We show that the ARF6 GTP/GDP cycle regulates the release of protease-loaded plasma membrane-derived microvesicles from tumor cells into the surrounding environment. To enable microvesicle shedding, ARF6-GTP-dependent activation of phospholipase D promotes the recruitment of the extracellular signal-regulated kinase (ERK) to the plasma membran

SUBMITTER: Muralidharan-Chari V 

PROVIDER: S-EPMC3150487 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature