Ontology highlight
ABSTRACT:
SUBMITTER: Hockemeyer D
PROVIDER: S-EPMC3152587 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Hockemeyer Dirk D Wang Haoyi H Kiani Samira S Lai Christine S CS Gao Qing Q Cassady John P JP Cost Gregory J GJ Zhang Lei L Santiago Yolanda Y Miller Jeffrey C JC Zeitler Bryan B Cherone Jennifer M JM Meng Xiangdong X Hinkley Sarah J SJ Rebar Edward J EJ Gregory Philip D PD Urnov Fyodor D FD Jaenisch Rudolf R
Nature biotechnology 20110707 8
Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALE ...[more]