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Bright and stable near-infrared fluorescent protein for in vivo imaging.

ABSTRACT: Imaging biological processes in mammalian tissues will be facilitated by fluorescent probes with excitation and emission bands within the near-infrared optical window of high transparency. Here we report a phytochrome-based near-infrared fluorescent protein (iRFP) with excitation and emission maxima at 690 nm and 713 nm, respectively. iRFP does not require an exogenous supply of the chromophore biliverdin and has higher effective brightness, intracellular stability and photostability than earlier phytochrome-derived fluorescent probes. Compared with far-red GFP-like proteins, iRFP has a substantially higher signal-to-background ratio in a mouse model due to its infrared-shifted spectra.

SUBMITTER: Filonov GS

PROVIDER: S-EPMC3152693 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Bright and stable near-infrared fluorescent protein for in vivo imaging.

Filonov Grigory S GS Piatkevich Kiryl D KD Ting Li-Min LM Zhang Jinghang J Kim Kami K Verkhusha Vladislav V VV

Nature biotechnology 20110717 8

Imaging biological processes in mammalian tissues will be facilitated by fluorescent probes with excitation and emission bands within the near-infrared optical window of high transparency. Here we report a phytochrome-based near-infrared fluorescent protein (iRFP) with excitation and emission maxima at 690 nm and 713 nm, respectively. iRFP does not require an exogenous supply of the chromophore biliverdin and has higher effective brightness, intracellular stability and photostability than earlie ...[more]

PMID: 21765402

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