Ontology highlight
ABSTRACT:
SUBMITTER: Ubhi D
PROVIDER: S-EPMC3152699 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Ubhi Devinder D Kavanagh Kathryn L KL Monzingo Arthur F AF Robertus Jon D JD
Archives of biochemistry and biophysics 20110612 1
Fungal methionine synthase, Met6p, transfers a methyl group from 5-methyl-tetrahydrofolate to homocysteine to generate methionine. The enzyme is essential to fungal growth and is a potential anti-fungal drug design target. We have characterized the enzyme from the pathogen Candida albicans but were unable to crystallize it in native form. We converted Lys103, Lys104, and Glu107 all to Tyr (Met6pY), Thr (Met6pT) and Ala (Met6pA). All variants showed wild-type kinetic activity and formed useful cr ...[more]