Ontology highlight
ABSTRACT:
SUBMITTER: Burke JE
PROVIDER: S-EPMC3155019 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Structure (London, England : 1993) 20110801 8
Phosphoinositide 3-kinase δ is upregulated in lymphocytic leukemias. Because the p85-regulatory subunit binds to any class IA subunit, it was assumed there is a single universal p85-mediated regulatory mechanism; however, we find isozyme-specific inhibition by p85α. Using deuterium exchange mass spectrometry (DXMS), we mapped regulatory interactions of p110δ with p85α. Both nSH2 and cSH2 domains of p85α contribute to full inhibition of p110δ, the nSH2 by contacting the helical domain and the cSH ...[more]