Ontology highlight
ABSTRACT:
SUBMITTER: Chapman JA
PROVIDER: S-EPMC3158087 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Chapman Jarrod A JA Ho Isaac I Sunkara Sirisha S Luo Shujun S Schroth Gary P GP Rokhsar Daniel S DS
PloS one 20110818 8
We describe a new algorithm, meraculous, for whole genome assembly of deep paired-end short reads, and apply it to the assembly of a dataset of paired 75-bp Illumina reads derived from the 15.4 megabase genome of the haploid yeast Pichia stipitis. More than 95% of the genome is recovered, with no errors; half the assembled sequence is in contigs longer than 101 kilobases and in scaffolds longer than 269 kilobases. Incorporating fosmid ends recovers entire chromosomes. Meraculous relies on an eff ...[more]