Inhibitory effect of (-)-epigallocatechin gallate on titanium particle-induced TNF-? release and in vivo osteolysis.

ABSTRACT: Tumor necrosis factor-? (TNF-?) and inflammatory cytokines released from activated macrophages in response to particulate debris greatly impact periprosthetic bone loss and consequent implant failure. In the present study, we found that a major polyphenolic component of green tea, (-)-epigallocatechin gallate (EGCG), inhibited Ti particle-induced TNF-? release in macrophages in vitro and calvarial osteolysis in vivo. The Ti stimulation of macrophages released TNF-? in a dose- and time-dependent manner, and EGCG substantially suppressed Ti particle-induced TNF-? release. Analysis of signaling pathway showed that EGCG inhibited the Ti-induced c-Jun N-terminus kinase (JNK) activation and inhibitory ?B (I?B) degradation, and consequently the Ti-induced transcriptional activation of AP-1 and NF-?B. In a mouse calvarial osteolysis model, EGCG inhibited Ti particle-induced osteolysis in vivo by suppressing TNF-a expression and osteoclast formation. Therefore, EGCG may be a potential candidate compound for osteolysis prevention and treatment as well as aseptic loosening after total replacement arthroplasty.

SUBMITTER: Jin S 

PROVIDER: S-EPMC3158500 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature