Dataset Information

Time-course analysis of DNA damage response-related genes after in vitro radiation in H460 and H1229 lung cancer cell lines.

ABSTRACT: Radiation is the most useful treatment modality for cancer patients. It initiates a series of signal cascades such as DNA damage response (DDR) signaling for repairing damaged DNA, arresting the cell cycle, and inducing cell death. Until now, few genes have been found to be regulated by radiation, which explains the molecular mechanisms of cellular responses to radiation. Although the transcriptional changes caused by radiation have been widely investigated, little is known about the direct evidence for the transcriptional control of DDR-related genes. Here, we examined the radiosensitivity of two non-small cell lung cancer cell lines (H460 and H1299), which have different p53 status. We monitored the time-dependent changes of 24 DDR-related gene expressions via microarray analysis. Based on the basal expression levels and temporal patterns, we further classified 24 DDR-related genes into four subgroups. Then, we also addressed the protein levels of several DDR-related genes such as TopBP1, Chk1 and Chk2, confirming the results of microarray analysis. Together, these results indicate that the expression patterns of DDR-related genes are associated with radiosensitivity and with the p53 statuses of H460 and H1299, which adds to the understanding of the complex biological responses to radiation.

SUBMITTER: Kim KH

PROVIDER: S-EPMC3158501 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Time-course analysis of DNA damage response-related genes after in vitro radiation in H460 and H1229 lung cancer cell lines.

Kim Kang Ho KH Yoo Hae Yong HY Joo Kyeung Min KM Jung Yong Y Jin Juyoun J Kim Yonghyun Y Yoon Su Jin SJ Choi Seung Ho SH Seol Ho Jun HJ Park Woong Yang WY Nam Do Hyun DH

Experimental & molecular medicine 20110701 7

Radiation is the most useful treatment modality for cancer patients. It initiates a series of signal cascades such as DNA damage response (DDR) signaling for repairing damaged DNA, arresting the cell cycle, and inducing cell death. Until now, few genes have been found to be regulated by radiation, which explains the molecular mechanisms of cellular responses to radiation. Although the transcriptional changes caused by radiation have been widely investigated, little is known about the direct evid ...[more]

PMID: 21633183

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Dataset Information

Time-course analysis of DNA damage response-related genes after in vitro radiation in H460 and H1229 lung cancer cell lines.

Publications

Time-course analysis of DNA damage response-related genes after in vitro radiation in H460 and H1229 lung cancer cell lines.

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